Journal: Cell Chemical Biology

Article Title: Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis

doi: 10.1016/j.chembiol.2021.05.019

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Article Snippet: Post antigen binding, the cells were washed once with ice-cold PBSB and incubated with mouse anti-Myc (1000x dilution) and chicken anti-His (1000x dilution; Invitrogen, PA1-9531) antibodies on ice for 20 min. Post primary incubation, the cells were washed with ice-cold PBSB and incubated with goat anti-mouse IgG AF488 (200x dilution) and donkey anti-chicken IgY F(ab’) 2 fragment AF647 (500x dilution; Jackson ImmunoResearch, 703-606-155) antibodies on ice for 4 min. Post-secondary incubation, the cells were washed once with ice-cold PBSB and evaluated by flow cytometry.

Techniques: Virus, Recombinant, Luciferase, Protease Inhibitor, DC Protein Assay, Plasmid Preparation, Software, Magnetic Beads, Gel Extraction, Purification

Journal: Cell reports

Article Title: Epithelial organoid supports resident memory CD8 T cell differentiation

doi: 10.1016/j.celrep.2024.114621

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Article Snippet: Goat anti-rabbit AF488 secondary , Jackson Immunoresearch , Cat#111–545-144, RRID:AB_2338052.

Techniques: Recombinant, Virus, Expressing, Cell Isolation, Comparison, Infection, Software

Journal: Cell reports

Article Title: Lung Mammary Metastases but Not Primary Tumors Induce Accumulation of Atypical Large Platelets and Their Chemokine Expression

doi: 10.1016/j.celrep.2019.10.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Goat anti-hamster, AF488 , Jackson ImmunoResearch , Cat# 127-545-160, RRID:AB_2338997.

Techniques: Recombinant, RNA Sequencing, Software